Journal: Journal of Materials Science. Materials in Medicine

Article Title: Copper tannic acid coordination nanosheet as a potent in-situ antibiotic sustained-release carrier for chronic osteomyelitis

doi: 10.1007/s10856-025-06979-z

Figure Lengend Snippet: The effect of Van@CuTA on BMSCs and HUVECs. A CCK-8 assay demonstrated no toxicity of Van@CuTA toward BMSCs. B Representative images of alizarin red staining, revealing increased calcium deposition in BMSCs treated with Van@CuTA. C , D Representative images and statistical analysis of HUVECs migration assay demonstrating a significant increase in migrated cells following Van@CuTA treatment. E – G Tube formation assay images and corresponding quantifications of total tube length and node number per view indicated that Van@CuTA significantly promoted HUVECs’ tube formation. (* indicated p < 0.05)

Article Snippet: Primary bone marrow mesenchymal stem cells (BMSCs) and Human Umbilical Vein Endothelial Cells (HUVECs) were obtained from ATCC.

Techniques: CCK-8 Assay, Staining, Migration, Tube Formation Assay

Journal: bioRxiv

Article Title: Matrix stiffness and stress relaxation regulate osteogenesis through histone demethylases KDM4B and KDM6B

doi: 10.1101/2025.07.16.665229

Figure Lengend Snippet: A , Schematic illustrating the approach to tuning alginate hydrogel mechanical properties. B , Measurements of hydrogel elastic modulus one day after crosslinking. C , D , Quantifications of stress relaxation half-times across hydrogel matrices. E , Representative images of alkaline phosphatase staining (left) indicating osteogenic differentiation of hBMSCs. Quantification of ALP-positive cells across hydrogel conditions (right). F , Gene expression data for early osteogenic markers from cells cultured across hydrogel conditions. G , Representative micrographs of cells cultured across hydrogel conditions stained for paxillin, β1 integrin, and nuclei (left) with quantifications of both paxillin intensity and colocalization of paxillin with β1 integrin (right). Statistical significance was determined by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple testing correction. n = 3 replicates per condition. * indicates p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Primary human BMSCs (hBMSCs) were obtained from Millipore Sigma (SCC034) and cultured according to established protocols ( Huebsch et al ., 2010 ; Darnell et al ., 2017 ).

Techniques: Staining, Gene Expression, Cell Culture

Journal: Advanced Healthcare Materials

Article Title: Development of a Microfluidic Vascularized Osteochondral Model as a Drug Testing Platform for Osteoarthritis

doi: 10.1002/adhm.202402350

Figure Lengend Snippet: Model characterization. a) Phase contrast photo of the cartilage and bone interface on day 1. b) Immunofluorescence image of the complete model on day 14 showing the different cell types stained in different colors. c) Collagen II expression in the cartilage compartment comparing DMEM‐based chondrogenic medium composition and EGM2‐based chondrogenic composition. d) Expression of the transcriptional factor SOX9 in chondrocytes in the presence of DMEM‐based chondrogenic medium. e) RANK expression in osteoclasts in the presence and in the absence of MCSF and RANKL. f) RANKL expression in the bone compartment. g) BMSCs and osteoblasts contributing to RANKL expression. h) TRAP and ALP enzymatic assays showing osteoclasts and osteoblasts activity in the selected bone medium composition. i) Undifferentiated BMSCs supporting the MVN (arrow 1) or committed to osteogenic lineage (arrow 2). j) Osteocalcin expression by differentiated BMSCs (arrow 1) and pre‐embedded osteoblasts (arrow 2).

Article Snippet: Osteoblasts were pre‐differentiated starting from human primary BMSCs purchased from Lonza (Cat. No. PT‐2501) expanded and frozen at passage 4, as described above.

Techniques: Immunofluorescence, Staining, Expressing, Activity Assay